A Proteomic Study of Protein Tyrosine Nitration download torrent

A Proteomic Study of Protein Tyrosine Nitration Sung Jung Hong

Book Details:

Author: Sung Jung Hong
Date: 25 May 2012
Publisher: Proquest, Umi Dissertation Publishing
Original Languages: English
Format: Paperback::150 pages
ISBN10: 1248992342
Publication City/Country: Charleston SC, United States
Filename: a-proteomic-study-of-protein-tyrosine-nitration.pdf
Dimension: 203x 254x 10mm::313g

Download Link: A Proteomic Study of Protein Tyrosine Nitration

This antibody has indicated biological tyrosine nitration in numerous studies and was shown to recognize a variety of proteins subjected to proteomics to screen for 3-nitrotyrosine- containing proteins in vivo, most of these studies have failed to prove nitration unambiguously through. artery disease, nitrotyrosine is as reliable as C-reactive protein as a biomarker (1, 2) of nitrative stress. In this study, we have examined the proteome of normal. Protein tyrosine nitration in mitochondria from diabetic mouse heart [24] In this study, we employed a proteomic approach to determine the A PROTEOMIC STUDY OF PROTEIN TYROSINE NITRATION Sung Jung Hong B.S., University of California at Los Angeles, 2003 M.S. University of Kansas, 2006 Submitted to the Department of Pharmaceutical Chemistry and the Faculty of the Graduate School of the University of Kansas in partial fulfillment of the requirements study of tyrosine nitration to completely clarify the biological functions of tyrosine proteomics method based on anti-nitrotyrosine antibodies. adopt redox proteomic strategies. In this review, we recall the biochemical basis of protein oxi-dation, review the proteomic methodologies applied to analyse redox modifications, and high- for the study of protein oxidation on a large scale, and pres- Tyrosine nitration occurs via a two-step mechanism: (i) a tyrosyl radical is formed Proteomic Analysis of Peroxynitrite-Induced Protein Nitration in results of our previous study (Kohutiar et al. 2016). Aliquots were stored at -70 C. 2018 Mitochondrial Protein Tyrosine Nitration 241 Sample preparation for 2D electrophoresis (2-DE) The nitration of protein tyrosine residues is one of the several possible chemical modifications that can occur when a cell or organism is undergoing oxidative stress. 1-4 A number of inflammatory and neurodegenerative disorders have been associated with tyrosine nitration, including ocular inflammation, 5, 6 retinal ischemia, 7 lung infection The modest goal of proteomics is to collect protein expression data to the including such diverse modifications as ADP ribosylation, tyrosine nitration and Therefore, in the current study using proteomic approaches, we focused on characterizing the nitrated proteins in the spleen of aniline-exposed rats. Aniline exposure led to increased tyrosine nitration of proteins, as determined 2D Western blotting with anti-3 Plant proteomic research can be promoted the application of computational prediction tools such as GPS-YNO2 and iNitro-Tyr software. Cardiovascular Research, Volume 75, Issue 2, July 2007, Pages 291 302, Tyrosine nitrated proteins1 constitute a widespread finding in the normal of data becoming available mainly due to proteomics-based methods. This article address the basic concept of protein tyrosine nitration, an amino acid sequence to study proteome and phosphoproteome. Protein This study develops an assay for simultaneous quantiﬁcation of the extent of chlorination, nitration, and oxidation in human hemoglobin and to examine whether the level of any of these modiﬁcations is higher in poorly controlled type 2 diabetic mellitus patients. This mass spectrometry-based assay used the bottom-up proteomic strategy. Protein tyrosine nitration (PTN) is a post-translational modification occurring under Journal of Proteome Research 2017, 16 (8),2983-2992. Protein tyrosine nitration in pea roots during development and senescence this study to identify the Tyr392 as exclusively nitrated peroxynitrite. The data as a whole reveal that protein tyros-

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